Investigation of the Effects of the Explant Type and Different Plant Growth Regulators on Micropropagation of Five Mediterranean Salvia spp. Native to Greece

Sages are medicinal and aromatic plants that constitute a large pool from which active compounds of great pharmaceutical potential can be derived, while at the same time, they also have ornamental value. The purpose this study was to develop micropropagation protocols Salvia fruticosa, S. officinalis, ringens, tomentosa, pomifera ssp. facilitate their exploitation in floriculture industries. In vitro cultures ringens studied for first time. Shoot tips single node explants seedlings were initially cultured on hormone free (Hf)-MS medium, followed by subcultures MS medium supplemented with 6-benzyladenine (BA) all species, as well zeatin (ZEA), kinetin (KIN), 6-(?,?-dimethylallyamino) purine (2iP), or meta-topolin (mT) fruticosa concentrations 0.0 3.2 mg L?1, combination 0.01 L?1 1-naphthaleneacetic acid (NAA). officinalis most efficient shoot multiplication species. highest indices found using 0.8 BA 0.4 BA, mT lower than other three Hyperhydricity problem stage, pronounced explants, increasing proportion cytokinin concentration. Microshoots rooted high percentages (75–85%) half-strength 0.5 Indole-3-butyric (IBA), except those best 1.0–2.0 IBA. Ex acclimatization highly successful (80–95%) peat–perlite substrate (1:1 v/v). Thus, present resulted five Mediterranean sage species native Greece, will breeding programs promotion these